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Bd Accuri C6 Flow Cytometer Software Download Free Updated FREE

Bd Accuri C6 Flow Cytometer Software Download Free

Table of Contents: BD Accuri C6 Laboratory Equipment Owner's Manual

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BD Accuri C6 BD Accuri C6 Flow Cytometer Instrument Manual 7820018

Rev-two 3 Refer to the BD Accuri C6 Safety and Limitations Manual (PN 7820091) for consummate safety data. When setting upward the cytometer, annotation the details in the post-obit sections to maximize the musical instrument'due south functioning. Operating temperature of the cytometer is between fifteen°C and 30°C (59°F and 86°F). Operating humidity is < eighty%

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BD Accuri C6 Flow Cytometer Instrument Manual ii

7820018-01 Rev-2 FIGURES AND TABLES Figure i-one. The BD Accuri C6 Flow Cytometer Organisation ............................................................. 1 Table i-three. BD Accuri C6 Flow Cytometer Specifications ............................................................ 2 Table ii-one. Canonical Reagents .....................................................................

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BD Accuri C6 Flow Cytometer Instrument Manual 7820018

Rev-ii 15  Click on the Unclog button in the Collect tab.  Select Instrument > Run Unclog Bicycle. For a thorough cleaning of the flow cell, perform an extended flow cell make clean. During extended menstruation jail cell cleaning, the period cell fills completely with cleaning solution from the sample tube on the SIP. This bicycle automatically shuts down the cytometer with cleaning reagent i

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BD Accuri C6 BD Accuri C6 Menstruum Cytometer Instrument Manual 7820018

Rev-2 9 Effigy 3-v. Proper Attachment of Fluidics Lines 1. Plug the cord from the power supply into the dorsum of the cytometer. Effigy three-half-dozen. Cable Connections 2. Adhere the black ability string to the ability supply and plug into a standard outlet. Only operate this musical instrument from a supply source that incorporates a tertiary-wire protective grounding con

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BD Accuri C6 BD Accuri C6 Menses Cytometer Instrument Manual 7820018

Rev-2 v Perform all of the procedures in this chapter earlier running any experimental samples. The cytometer is equanimous of fluidics, optics, and electronics subsystems that work together to analyze cell populations. The following figures show the major components of the fluidics and optics subsystems. Figure iii-1. BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Li

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BD Accuri C6 BD Accuri C6 Flow Cytometer Instrument Transmission 18

7820018-01 Rev-2 half dozen. Place a tube with ii mL of 0.22 µm filtered, DI h2o on the SIP and run for i infinitesimal. Check the cytometer periodically to ensure there are no fluid leaks or crimped lines. one. Plough off the cytometer. ii. Open up the elevation embrace. 3. Visually inspect for fluid leaks by looking for pocket-sized pools of liquid well-nigh whatsoever of the visible luer

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BD Accuri C6 BD Accuri C6 Flow Cytometer Instrument Manual 7820018

Rev-2 17 Figure 4-2. Waste material Line Quick Disconnect 2. Remove the bottle lid. iii. Dispose of waste material according to local regulations. 4. Add approximately 100 mL of 0.five% NaOCl to the Waste material bottle. five. Supersede the lid. 6. Reattach the waste line to the top of the bottle. Before turning on the cytometer, visually check all the bottles and fill the sheath

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BD Accuri C6 Period Cytometer Instrument Manual 7820018

Rev-2 i TABLE OF CONTENTS 1 INTRODUCTION ................................................................................................................. 1 1.1 Near This Manual ..................................................................................................... 1 one.2 Delivery and Inspection ..................................................

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BD Accuri C6 BD Accuri C6 Flow Cytometer Musical instrument Manual 2

7820018-01 Rev-2 Table 1-3. BD Accuri C6 Menses Cytometer Specifications Laser Excitation 488 nm (rated at xx,000 hr life) 640 nm (rated at twenty,000 hr life) Light amplification by stimulated emission of radiation Profile 10 ten 75 µm Laser Power fourteen.7 mW 640 nm Diode Scarlet Light amplification by stimulated emission of radiation 20 mW 488 nm Solid State Blue Laser Besprinkle Detection Forward (0 degrees, +/-13) Side (90 degrees, +/-13) Emission Detection iv colors, sta

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BD Accuri C6 Flow Cytometer Instrument Transmission 7820018

Rev-2 vii 6. Select "Apply," then "OK" to save changes and close dialog box. one. Remove the cytometer and other components from the shipping boxes. two. With the forepart of the cytometer facing toward you, lift the top cover. three. Verify that the in-line sheath filter is installed. If it is non installed, meet section iv.eleven for instructions on replacing the filter. F

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BD Accuri C6 BD Accuri C6 Menses Cytometer Musical instrument Manual 7820018-01

Rev-2 i The BD Accuri ™ C6 catamenia cytometer offers an entirely new vision for the role of menstruum cytometry in life science inquiry. Designed from the ground up to be compact and easy to apply, the BD Accuri C6 flow cytometer fits in whatsoever lab and is readily attainable to all researchers—experts and novices akin. Figure 1-1. The BD Accuri C6 Flow Cytometer Sys

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BD Accuri C6 BD Accuri C6 Flow Cytometer Instrument Manual 7820018

Rev-ii 27 Visit world wide web.AccuriCytometers.com or www.BDBiosciences.com for a complete listing of parts and reagents. Regional Offices bdbiosciences.com/offices Asia Pacific Australia/New Zealand Europe Singapore Australia BD Biosciences Tel 65.6861.0633 Cost Free: 1800 656 100 Tel 32.ii.400.98.95 Fax 65.6860.1590 Tel 61.2.8875.7000 F

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BD Accuri C6 BD Accuri C6 Flow Cytometer Instrument Manual 7820018

Rev-2 25 2. Identify a sample tube containing 3 mls of diluted Decontamination Fluid (PN 653154) on the SIP and run the instrument for 5 minutes on FAST. 3. Place a sample tube containing iii mls of 0.22 µm filtered, DI water on the SIP and run the instrument for 2 minutes on FAST. 4. From the Instrument menu in BD Accuri C6 Software, activate the manual decontamination fluid cycle past sele

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BD Accuri C6 BD Accuri C6 Menses Cytometer Instrument Manual 20

7820018-01 Rev-2 5. Pull the luer lock connectors outward and slide the luer lock fittings off the pump head. Figure 4-6. Sliding Luer Lock Fittings Off of Pump Head half dozen. Remove the peristaltic pump tubing and discard according to standard laboratory protocols and regulations. 7. Replace with new peristaltic pump tubing (PN 653146) by sliding the lu

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BD Accuri C6 Period Cytometer Musical instrument Manual 24

7820018-01 Rev-two i. Pause and restart the sample. two. Run a backflush or unclog (encounter sections four.ii and iv.3.1). 3. Run the validation beads (PN 653144 or 653145) (see the BD Accuri C6 Software User Guide for details). If the beads expect acceptable, there may be a problem with the sample. If the beads are out of operation specification, perform a decontamination fluid cycle (see sect

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BD Accuri C6 Flow Cytometer Instrument Manual 7820018

Rev-2 23 Figure five-3. Windows Security Window  Windows will start the installation of the driver for the C6 cytometer. In one case completed, you have successfully installed the driver software. When prompted, click the Close button. Figure 5-4. Update Driver Software – Cytometer, Successfully updated your driver software  With the C6 commuter installed and the USB c

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BD Accuri C6 BD Accuri C6 Period Cytometer Instrument Manual 8

7820018-01 Rev-2 vi. Gently close the cytometer cover. Make certain the cover is securely positioned. If the cover is not closed deeply, the cytometer will not function. 7. Identify the fluidics canteen tray in a convenient location near the perimeter of the cytometer. Do not place the fluidics bottle tray on summit of the cytometer. 1. Fill the sheath bottle with sheath fluid. two.

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BD Accuri C6 BD Accuri C6 Flow Cytometer Instrument Transmission vi

7820018-01 Rev-2 Install the software on the computer before connecting the USB cable to the cytometer. NOTE: We recommend making the following changes to the C6 workstation figurer in guild to achieve the best possible work surround and to avert unexpected software computer bug that could potentially upshot in a loss of data. three.two.2 Changing the Workstation Compute

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BD Accuri C6 Flow Cytometer Instrument Transmission 10

7820018-01 Rev-ii This section provides details on installing BD Accuri C6 Software and the BD Accuri C6 flow cytometer driver. In most cases, BD Accuri C6 Software will be pre-installed. If software is installed, skip to department three.5. 1. Ensure the USB cablevision is not connected between the computer and cytometer. two. Start up the computer and monitor. 3. Insert the provided BD Accu

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BD Accuri C6 BD Accuri C6 Flow Cytometer Instrument Manual 7820018

Rev-ii eleven 4. Firmly printing the power button on the forepart of the unit to turn on the cytometer. During start up, the BD Accuri C6 Software Traffic Calorie-free turns yellow and the cytometer pumps starting time to run. When powered on, the cytometer automatically flushes the fluidics lines with sheath. This process takes approximately 5 minutes. Exercise non open the hat of the cytometer duri

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BD Accuri C6 BD Accuri C6 Menstruum Cytometer Instrument Manual four

7820018-01 Rev-2 To obtain an MSDS, visit www.AccuriCytometers.com or contact:  In the USA: Email [email protected] Call Accuri Technical Support at 1.734.994.8000  Outside the USA Visit www.AccuriCytometers.com for country-specific contact information.

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BD Accuri C6 Menses Cytometer Instrument Manual 22

7820018-01 Rev-ii  Navigate to the location of the commuter software and specify the following path: C:\Programme Files\BD Accuri\BD Accuri C6 Software\libraries And then click on the OK button to enter the selected path into the Scan window. Figure five-ane. Browse For Folder Dialog Box  Click the Next push button. Figure 5-2. Update Driver Software – Cytometer, Sec

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BD Accuri C6 BD Accuri C6 Menses Cytometer Instrument Transmission 14

7820018-01 Rev-2 BD Accuri C6 Software will trigger the notification based on the book of sheath run through the organisation or on the date since maintenance was last performed. BD Accuri C6 Software will brandish this notification based on whichever trigger is reached first. The notification appears equally a pop-up window that allows the user to choose between 'Remind me later on' o

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BD Accuri C6 Flow Cytometer Musical instrument Manual 7820018

Rev-two 13 Changing the in-line sheath filter, fluidic bottle filters, and peristaltic pump tubing every two months is recommended. Heavy utilize of the cytometer may warrant more frequent changes. The post-obit table lists the parts and reagents that are required for regular maintenance. Table 4-1. BD Accuri C6 Flow Cytometer Replacement Parts/Reagents Listing Office Number

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Bd Accuri C6 Flow Cytometer Software Download Free

DOWNLOAD HERE

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